Abstract
Circulating tumor cells (CTCs) are rare cells existing in the bloodstream with a relatively low number, which facilitate as a predictor of cancer progression. However, it is difficult to obtain highly purified intact CTCs with desired viability due to their low percentage among blood cells. In this chapter, we demonstrate the detailed steps for the fabrication and application of the novel self-amplified inertial-focused (SAIF) microfluidic chip that enables size-based, high-throughput, label-free separation of CTCs from the patient blood. The SAIF chip introduced in this chapter demonstrates the feasibility of an extremely narrow zigzag channel (with 40 μm channel width) connected with two expansion regions to effectively separate different-sized cells with amplified separation distance.