Re-scan confocal microscopy: scanning twice for better resolution

重新扫描共聚焦显微镜:扫描两次以获得更好的分辨率

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作者:Giulia M R De Luca, Ronald M P Breedijk, Rick A J Brandt, Christiaan H C Zeelenberg, Babette E de Jong, Wendy Timmermans, Leila Nahidi Azar, Ron A Hoebe, Sjoerd Stallinga, Erik M M Manders

Abstract

We present a new super-resolution technique, Re-scan Confocal Microscopy (RCM), based on standard confocal microscopy extended with an optical (re-scanning) unit that projects the image directly on a CCD-camera. This new microscope has improved lateral resolution and strongly improved sensitivity while maintaining the sectioning capability of a standard confocal microscope. This simple technology is typically useful for biological applications where the combination high-resolution and high-sensitivity is required.

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