Abstract
Prolamin and glutelin are the major constituents of rice protein. The rapid and non-destructive detection of prolamin and glutelin content is conducive to the accurate assessment of rice quality. In this study, hyperspectral imaging combined with regression models and feature wavelength selection was employed to detect the rice prolamin and glutelin content. Feature wavelength selection was achieved using the successive projections algorithm (SPA), competitive adaptive reweighted sampling (CARS), and convolutional neural network (CNN)-based Gradient-weighted Class Activation Mapping++ (GradCAM++). Partial least squares regression (PLSR), support vector regression (SVR), back-propagation neural network (BPNN), and CNN models were established using the full spectra and the feature wavelengths. The BPNN models showed the best prediction performance for prolamin and glutelin. The optimal BPNN models achieved a correlation coefficient (r) greater than 0.8 for both proteins. Performance differences were observed between models using feature wavelengths and those using the full spectra. The GradCAM++ method was used to select feature wavelengths with different threshold values, and the performance of different threshold values were compared. The results demonstrated that hyperspectral imaging with multivariate data analysis was feasible for predicting the rice prolamin and glutelin content. This study provided a methodological reference for detecting prolamin and glutelin in rice, as well as the other protein types.