Two-Color Total Internal Reflection Fluorescence Microscopy of Exocytosis in Endocrine Cells
双色全内反射荧光显微镜观察内分泌细胞的胞吐作用
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作者:Adam J Trexler, Justin W Taraska
Abstract
We describe a comprehensive method for imaging and analysis of local protein dynamics at single sites of exocytosis in living cultured endocrine cells. This method is well suited to quantitatively map the complex dynamics of individual molecules at single sites of vesicle fusion in live cells.
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