Molecular identification of Cucumber mosaic virus isolates of subgroup IB associated with mosaic disease of eggplant in India

与印度茄子花叶病有关的黄瓜花叶病毒IB亚群分离物的分子鉴定

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作者:Susheel Kumar, Karmveer Kumar Gautam, Shri Krishna Raj

Abstract

Association of Cucumber mosaic virus (CMV) with severe mosaic disease of eggplant (Solanum melongena L.) collected from Lucknow and Kanpur, India was initially detected by host reaction and serological assay and confirmed by RT-PCR employing coat protein gene specific primers. Further, molecular identification of the virus isolates was done by cloning and sequence analysis of the complete RNA3 genome. Based on 97-99 % identities and phylogenetic relationships, the virus isolates infecting eggplant were identified as members of CMV subgroup IB.

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