Abstract
BACKGROUND: For many microbial processes, the complexity of the metabolisms and the responses to transient and realistic conditions are difficult to capture in mechanistic models. The cells seem to have an innate intelligence that enables them to respond optimally to environmental changes. Some "intelligent" models have therefore been proposed and compared with a mechanistic model for fed-batch cultures of Ralstonia eutropha. RESULTS: Two kinds of models have been proposed to describe such cellular behavior. Cybernetic models are derived through postulates of cellular intelligence and memory, and neural models use artificial intelligence through neural networks. Some competing models of both kinds have been compared for their ability to portray and optimize the synthesis of poly-beta-hydroxybutyrate by Ralstonia eutropha in fed-batch cultures with finite dispersion. Neural models enabled the formation of more of the polymer than cybernetic models, with lesser utilization of the carbon and nitrogen substrates. Both types of models were decidedly superior to a mechanistic model used as a reference, thus supporting the value of intelligent descriptions of microbial kinetics in incompletely dispersed bioreactors. CONCLUSION: Neural and cybernetic models describe and optimize unsteady state fed-batch microbial reactors with finite dispersion more effectively than mechanistic models. However, these "intelligent" models too have weaknesses, and hence a hybrid approach combining such models with some mechanistic features is suggested.