Abstract
Understanding the mechanisms of action of adjuvants at the tissue level is crucial to the development of more potent and safer versions for human use. Comparative tissue proteomics presents a novel tool to study their unique action mechanisms. Here, we present a protocol for preparing murine tissue for comparative proteomics study of vaccine adjuvant mechanisms. We describe steps for adjuvant treatment in live animals, tissue harvesting, and homogenization. We then detail protein extraction and digestion to prepare for liquid chromatography-tandem mass spectrometry analysis. For complete details on the use and execution of this protocol, please refer to Li et al.1.