Abstract
Responses of broiler chickens to dietary glycine equivalent (Gly(equi)) are affected by dietary cysteine and choline. Hence, this study investigated interactive effects among dietary Gly(equi), cysteine, and choline on the growth of broiler chickens. Male Ross 308 broiler chickens were maintained in 105 metabolism units (10 birds/unit) from days 7 to 22. Excreta were collected in 12-h intervals from days 18 to 21. Blood was sampled on day 22 (1 bird/unit). Five levels each of Gly(equi) (9-21 g/kg), cysteine (2-5 g/kg), and choline (0.5-1.7 g/kg) were tested under 15 diets in 7 replicates each following a fractional central composite design. Another diet was provided to five metabolism units (15 birds/unit) to measure prececal amino acid digestibility. Data were evaluated using neural networks. The gain:feed ratio (G:F) increased with digestible Gly(equi) intake. Differences between low and high digestible cysteine intake were low. Effects of choline intake on G:F were low. Nitrogen-utilization efficiency (NUE) was high (≥77%), with low variation among treatments. Plasma metabolites varied among treatments and indicated that metabolism of Gly(equi), cysteine, and choline was influenced. These findings showed that interactive effects of dietary Gly(equi), cysteine, and choline on growth were small, possibly because NUE was barely influenced.