Linking Epitope-Specific T-Cell Receptors to IFNγ Secretion Using Nanovial Technology

To link IFNγ-secretion levels of epitope-specific T-cells with their TCRαβ, we coated nanovials with pHLA-I to capture and activate epitope-specific T-cells and their secreted IFNγ, followed by index-sorting and TCRαβ sequencing. We demonstrate that nanovials are a promising tool to link single epitope-specific TCRαβ clonotypes to the cell's functional properties.