Production of various organic acids from different renewable sources by immobilized cells in the regimes of separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SFF)

通过固定化细胞在单独水解发酵 (SHF) 和同时糖化发酵 (SFF) 模式下从不同的可再生资源中生产各种有机酸

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作者:Olga Maslova, Nikolay Stepanov, Olga Senko, Elena Efremenko

Abstract

The study was aimed at production of different organic acids (OA) (lactic, fumaric, or succinic) by various microbial cells (filamentous fungi Rhizopus oryzae (F-814, F-1127) and bacteria Actinobacillus succinogenes B-10111) immobilized into poly(vinyl alcohol) (PVA) cryogel from diverse renewable raw materials (wheat and rice straw, aspen and pine sawdust, Jerusalem artichoke stems and tubers, biomass of macro- and microalgae) under batch conditions. The process productivity, bulk output and OA concentrations were higher in case of using immobilized cells than in case of free cells under identical conditions. A higher OA productivity was reached via simultaneous enzymatic saccharification and microbial fermentation (SSF) of same raw materials as compared to their separate enzymatic hydrolysis and fermentation of accumulated reducing sugars (SHF). Maximal concentrations of all OAs studied were obtained for bioconversion of Jerusalem artichoke tubers. The immobilized cells were used in long-term conversion of various renewable materials to OAs in SSF.

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