Abstract
Biodegradable films were created with fish gelatin and fucoidan extracted from Sargassum tenerrimum using 30% glycerol as a plasticizer. The gelatin films were incorporated with fucoidan (2.5%, 5%, 7.5%, and 10%), respectively. Results presented that the average thickness of films ranged from 0.12 to 0.147 mm. Tensile strength (TS) was decreased from 29.27 to 3.46 MPa by adding the fucoidan except for the gelatin/fucoidan 10% (5.35 MPa) sample. The results showed that the physical characteristics (the contact angle (Ɵ), water solubility, opacity, and moisture content) of the films significantly changed depending on different fucoidan concentrations. FTIR and SEM analysis confirmed the interaction of fucoidan with gelatin in the composite film. Furthermore, adding 10% fucoidan showed high DPPH radical scavenging activity (65%) than other treatments. Therefore, incorporation of fucoidan extracted from brown algae (Sargassum tenerrimum) with fish gelatin films improved thermal stability, anti-oxidative, and antibacterial characteristics in addition to enhanced mechanical and protective properties, to be used as a bioactive edible film in the food packaging industry.