Genotyping of 44 isolates of Fusarium solani, the main agent of fungal keratitis in Brazil

对巴西真菌性角膜炎主要病原体——茄病镰刀菌(Fusarium solani)的44株分离株进行基因分型

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Abstract

In a retrospective study performed over 6 years in Brazil, Fusarium solani was found to be the most common species causing mycotic keratitis. The genetic diversity of 44 isolates from 39 patients was assessed by enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) and PCR restriction fragment length polymorphism (PCR-RFLP) fingerprinting. ERIC-PCR was more discriminatory than PCR-RFLP for differentiating the strains. By combining of the results of both techniques, we identified 40 genotypes. Molecular typing revealed a high genomic heterogeneity of the strains of F. solani studied.

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