Abstract
It is important to monitor the level of parasitic loads in herds of European bison and to identify threats early enough to prevent their spread to other populations or species. The aim of the present study was to compare the detection sensitivity of two fecal flotation techniques, viz. the modified Willis method (WM) with centrifugation and modified McMaster flotation technique (MM), in the diagnostics of gastrointestinal parasites of European bison before the translocation of animals. Out of 166 feces samples, Eimeria spp. oocysts (84.3% in WM and 71.1% in MM) and Trichostrongylidae eggs (82.5% in WM and 53.6% in MM) predominated. These were accompanied by eggs from Capillaria spp. (prevalence: 13.9% in WM and 3.61% in MM), Nematodirus spp. (prevalence: 18.1% in WM and 4.8% in MM) and Trichuris spp. (prevalence: 12.7% in WM and MM) were identified. The lowest prevalence was noted for cestode eggs of Moniezia spp. (5.4% in WM and 3.0% in MM). The Willis method yielded a higher prevalence of eggs and oocysts than the modified McMaster method, and hence has a higher probability of detecting parasitic structures than the modified McMaster method, especially in cases of very low levels of invasion. As the two methods yield consistent results, it is recommended to use the Willis method for diagnosis of internal parasite infection in European bison. This test offers more sensitive method than McMaster technique of detecting the presence of low levels of a variety of parasite eggs and oocysts in feces, while also being inexpensive and adaptable to field work.