Velocity-resolved 3D retinal microvessel imaging using single-pass flow imaging spectral domain optical coherence tomography

利用单次通过流成像光谱域光学相干断层扫描技术进行速度分辨三维视网膜微血管成像

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Abstract

We demonstrate in vivo velocity-resolved, volumetric bidirectional blood flow imaging in human retina using single-pass flow imaging spectral domain optical coherence tomography (SPFI-SDOCT). This technique uses previously described methods for separating moving and non-moving scatterers within a depth by using a modified Hilbert transform. Additionally, a moving spatial frequency window is applied, creating a stack of depth-resolved images of moving scatterers, each representing a finite velocity range. The resulting velocity reconstruction is validated with and strongly correlated to velocities measured with conventional Doppler OCT in flow phantoms. In vivo velocity-resolved flow mapping is acquired in healthy human retina and demonstrate the measurement of vessel size, peak velocity, and total foveal blood flow with OCT.

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