Abstract
As members of the Tenuipalpidae family, Brevipalpus mites have emerged as significant research subjects in agriculture and horticulture due to their polyphagous nature, high reproductive potential, and ability to transmit plant viruses. However, the evolutionary understanding of this taxon remains constrained by the absence of high-quality chromosomal genome data. In this study, we present the first chromosome-level genome assembly of B. obovatus, achieved through an integrative approach utilizing Illumina, Nanopore, PacBio, and Hi-C sequencing technologies. The final genome assembly comprises 67.28 Mb in length with contig and scaffold N50 values of 33.74 Mb and 33.74 Mb, respectively. The chromosome-scale assembly (five contigs anchored to two chromosomes) showed 87.4% BUSCO completeness (arachnida_odb12, n = 1,123). We annotated 9,193 protein-coding genes (89.1% BUSCO completeness), including 8,756 functionally annotated genes, and identified repetitive sequences accounting for 12.2%. This genome provides a valuable resource for advancing the understanding of flat mite genetics and evolution.