Abstract
Carpoglyphus lactis is a globally distributed stored-product pest mite that inflicts substantial damage on dried fruits, grains, and other preserved commodities. However, the absence of high-quality genomic resources has long constrained insights into its biology and evolutionary characteristics. In this study, we report a chromosome-level genome assembly for C. lactis, generated using an integrative approach that combines Illumina, PacBio, Oxford Nanopore, and Hi-C sequencing technologies. The assembled genome comprises approximately 53.47 Mb, featuring a contig N50 of 713.31 kb and a scaffold N50 of 5.92 Mb. Utilizing Hi-C data, 99.8% of the genome sequences were anchored onto nine chromosomes. Gene annotation revealed a total of 10,330 protein-coding genes, with repetitive sequences accounting for 9.66% of the genome. BUSCO completeness assessment using the arachnida_odb12 database (n = 1,123) indicated high integrity for both the genome assembly (94.6%) and gene annotation (94.7%). This chromosome-level genome serves as a valuable resource for investigating mite biology and facilitating the development of effective control strategies against this economically important pest species.