Abstract
Ralstonia solanacearum is one of the major plant pathogens causing bacterial wilt disease in a variety of plant species. In Vietnam, according to our knowledge, we first discovered R. pseudosolanacearum, which is one of four phylotypes of R. solanacearum, as a causal agent wilting in cucumber (Cucumis sativus). Due to the latent infection of R. pseudosolanacearum and its heterogenous species complex, controlling the disease becomes difficult.Therefore, the study of R. pseudosolanacearum has great significance to generate effective disease management and treatment. Here, we assembled the isolate R. pseudosolanacearum strain T2C-Rasto, which possessed 183 contigs with 67.03% GC content of 5,628,295 bp in. This assembly included 4,893 protein sequences, 52 tRNA genes, and 3 rRNA genes. In addition, the virulence genes involved in the colonization of the bacterium and wilting to the host were defined in twitching motility (pilT, pilJ, pilH and pilG), chemotaxis (cheA and cheW), type VI secretion system (ompA, hcp, paar, tssB, tssC, tssF, tssG, tssK, tssH, tssJ, tssL and tssM), type III secretion system (hrpB and hrpF).