Abstract
Apple stem pitting virus (ASPV) mainly infects apple, pear and their closely related species in the world. ASPV causes some symptoms like leaf etiolation and stony pit in cultivated pear, but produces few symptoms in cultivated apple. We inoculated tobacco with ASPV, which originates from Nanking cherry (Prunus tomentosa), and we obtained tobacco RNA-sequencing data through high-throughput sequencing. In total, 17,401,736 clean reads were obtained after sequencing and removing adaptor sequences, contamination and low-quality reads. An RNA-seq data has been uploaded to Sequence Read Archive (SRA), which enables researchers to access the RNA-sequencing data of tobacco inoculated with ASPV.