Abstract
Neutralizing monoclonal antibodies against poliovirus type 1 were obtained after conventional immunization or combined in vivo-in vitro immunization. Antibody binding sites were determined by sequence analysis of neutralization-resistant mutants. Site 3 variants had several amino acid substitutions in previously unidentified positions for neutralization resistance. Evidence for a linkage of subsites 3a and 3b is presented. Some site 3b antibodies as defined previously precipitated 14S subunits, although with reduced titers.