Characterization of an induced pluripotent stem cell line (NCHi013-A) from a 5-year-old male with pulmonary atresia with intact ventricular septum and a biventricular repair

一名患有肺动脉闭锁且室间隔完整并接受双心室修复的 5 岁男性的诱导性多能干细胞系 (NCHi013-A) 的特征

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作者:Meghan Hanley, Matthew Alonzo, Shiqiao Ye, Yang Yu, Javier Contreras, Jade Hayden, Vidu Garg, Ming-Tao Zhao

Abstract

Pulmonary atresia with intact ventricular septum (PA/IVS) is a rare congenital heart defect that causes a significant decrease of blood outflow from the heart and is fatal if left untreated. iPSC line NCHi013-A was produced from peripheral blood mononuclear cells from a male child with PA/IVS using Sendai virus reprogramming. NCHi013-A displayed normal stem cell morphology, expressed markers for pluripotency, and presented ability to differentiate into cells of endoderm, ectoderm, and mesoderm lineages. The iPSC line also maintained normal karyotype, was validated for cell identity, and tested negative for transgenes and mycoplasma contamination.

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