Aim
The effectiveness of PAW for aflatoxin B1 (AFB1), ochratoxin A (OTA), and fumonisin B1 (FB1) detoxification in naturally contaminated poultry feeds with its impacts on the feed quality were inspected.
Background
Plasma-activated water (PAW) is considered one of the emerging strategies that has been highlighted recently in the food industry for microbial decontamination and mycotoxin detoxification, due to its unique provisional characteristics.
Conclusion
The possibility of PAW for degrading more than a quarter to a third of the original quantity of targeted mycotoxins in poultry feeds after 10 minutes of treatment with a slight effect on feed quality.
Methods
PAW-30 and PAW-60 were utilized for feed treatment for six time durations (5, 10, 15, 20, 40, and 60 minutes) each. The alterations in the physicochemical properties of PAW after different time durations of plasma inducement and treatment with and without feed samples were monitored. Competitive enzyme-linked immunosorbent assay (ELISA) was employed for estimation of mycotoxin levels and high performance liquid chromatography (HPLC) was utilized for
Results
The physicochemical properties of PAW showed a significant decrease (p < 0.05) in pH value from 6.72 to 2.68 and a significant increase (p < 0.05) in oxidation-reduction potential (ORP), electrical conductivity (EC), and temperature from 235 mV, 5.1 μS/cm, and 20.5°C to 499.2 mV, 727.6 μS/cm, and 26.8°C, respectively, after 60 minutes of plasma inducement in a time-dependent manner. The mycotoxins decay kinetics after PAW application were illustrated. Mycotoxins degradation efficiency significantly increased (p < 0.05) with increasing water activation time. A significant increase (p < 0.05) in AFB1, OTA, and FB1 degradation levels was reported mainly during the first 10 minutes of treatment for AFB1 and the first 15 minutes for OTA and FB1 to record values of 28.33%, 32.14%, and 34.62% and 33.80%, 40.70%, and 43.38% after 60 minutes of feed exposure to PAW-30 and PAW-60, respectively. Significant differences (p < 0.05) between examined mycotoxins in their degradation levels were recorded, where FB1 exhibited the highest degradation levels. Generally, feed compositions were slightly affected by PAW and fats were still having good quality.