Abstract
The bone is composed of solid cortical bone and honeycomb-like trabecular bone. Although the cortical bone provides the substantial mechanical strength of the bone, few studies have focused on its regeneration. As the structural and functional units of the cortical bone, osteons play critical roles in bone turnover. Composed of osteocytes, lamellae, lacunocanalicular network, and Haversian canals, osteons exhibit a delicate and hierarchical architecture. Studies have attempted to reconstruct the osteonal structure with artificial approaches; however, hardly the four elements were recapitulated simultaneously. In this work, a series of bioengineering techniques, including electrospinning, micropatterning, and laser-directed microfabrication, were employed to develop a three-dimensional scaffolding system, which successfully recapitulated the osteon structure in vitro. The physiological morphology and bioactivity of osteocytes were emulated, the intercellular communications between osteocytes were identified, and the concentric lamellae and Haversian canals were simulated as well. This work constructed an in vivo-like platform for osteon study, providing convenience for exploring the interaction among the osteonal elements.