Synthesis of the Staphylococcus aureus Strain M Capsular Polysaccharide Repeating Unit

金黄色葡萄球菌M株荚膜多糖重复单元的合成

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Abstract

The synthesis of the Staphylococcus aureus strain M capsular polysaccharide repeating unit is reported. A postglycosylation oxidation strategy was utilized for the construction of the α-galactosaminuronic acid linkages, relying on a stereoselective 2-azido-4,6-O-di-tert-butylsilylidene galactopyranoside donor, for which the selectivity was assessed by model glycosylations. The α-fucosamine linkage was installed stereoselectively, using a reactive 2-azidofucosyl donor. An unexpected glycosidic bond cleavage during the TEMPO/PhI(OAc)(2)-mediated oxidation of a disaccharide intermediate was circumvented by a TEMPO/PhI(OAc)(2)-Pinnick oxidation protocol.

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