Abstract
To date, the preparation of a novel ultrafiltration membrane and the efficient separation and purification of protein solutions have gradually attracted widespread attention of many researchers. In this study, a hollow porous polystyrene/ethylene-vinyl acetate copolymer blend nanofibrous membrane (PS/EVA-BNM) was generated by electrospinning and chemical modification and then used to separate and purify proteins in solution. The BNM was characterized by scanning electron microscopy and specific surface area and pore size analyses. The membrane separation system was assembled using the BNM, which was overlaid to form the reaction layer. The optimal conditions for protein separation were determined by adjusting the operating pressure, filtration time, and pH. The results showed that the rejection rate of serum albumin and the membrane flux could reach 94.35% and 2.04 L/(m(2) min), respectively, under the following conditions: the operating pressure was 0.10 MPa and the processing time was 1.5 h. By comparing the parameters of the polyethersulfone commercial ultrafiltration membrane with the PS/EVA-BNM system, it could be inferred that the rejection rate of the latter decreased slightly, whereas its transport flux improved several times. At the same time, the experimental results indicated that the PS/EVA-BNM possessed excellent reusability and mechanical properties. Additionally, the BNM could retain its nanofibrous morphological structure after the separation of serum albumin several times in an aqueous environment.