Improvement of transduction efficiency of recombinant adeno-associated virus vector by entrapment in multilamellar liposomes

通过多层脂质体包裹提高重组腺相关病毒载体的转导效率

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Abstract

Recombinant adeno-associated virus (AAV) has attracted considerable interest as a potential vector for human gene therapy, but its transduction efficiency is quite low. The present study demonstrated AAV vector-associated liposomes to be more effective for in vitro gene transfer to human glioma cells than are liposomes containing plasmid DNA. Using vector-associated liposomes increased the transduction efficiency more than 10-fold compared to liposomes containing plasmid DNA and more than 6-fold compared to AAV alone. From these results, AAV vector-associated liposomes appear to be a good candidate for in vivo gene delivery to human gliomas.

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