Abstract
In recent years, mammal-derived extracellular vesicles (EVs) have been widely used in studies on tissue repair and antiaging. Their therapeutic potential lies in mediating intercellular communication through the transfer of various bioactive molecules. As research on nanovesicles progresses, plant-derived nanovesicles (PDNVs) have attracted growing attention as a promising alternative. As an emerging cross-species regulatory "natural force", PDNVs have attracted considerable interest due to their excellent biocompatibility, low immunogenicity, and remarkable therapeutic effects in tissue injury and aging-related diseases. In this review, we examine the bioactive components, drug delivery potential, and functional mechanisms of PDNVs, and we summarize recent advances in their applications for tissue repair and antiaging. In addition, we systematically discuss the major challenges and limitations hindering the clinical translation and industrialization of PDNVs, and we propose five strategic approaches along with future research directions. This review aims to promote further investigation of PDNVs in regenerative medicine and enhance their potential for clinical application.