First identification of Tusavirus in calf intestinal tissue suggests interspecies transmission and genomic variation

• This is the first identification of Tusavirus in calf tissue in China, expanding its host species and geographical range. • Genomic analysis reveals conservation, with nucleotide insertions in the VP1 gene and deletions in both VP1 and VP2 genes. • Phylogenetic analysis shows close evolutionary linkage between bovine and ovine TuV strains.