Abstract
Acquired immunodeficiency syndrome is a systemic infectious disease caused by human immunodeficiency virus infection, which could attack the bones and heart. However, the relationship between Nuclear Complex Associated 3 Homolog (NOC3L) and DEAD box helicase 17 (DDX17) and acquired immunodeficiency complicated with viral myocarditis and osteoporosis is unclear. The acquired immune deficiency dataset GSE140713, GSE147162 and the osteoporosis dataset (GSE230665), and viral myocarditis dataset (GSE150392) configuration files were generated from gene expression omnibus. The differentially expressed genes (DEGs) were screened and performed weighted gene co-expression network analysis. Construction and analysis of protein-protein interaction network. Functional enrichment analysis, gene set enrichment analysis, immune infiltration analysis, gene expression heatmap, and comparative toxicogenomics database analysis were performed. TargetScan screens miRNAs of DEGs. Thousand three hundred thirty-five DEGs were identified. According to gene ontology, they are mainly concentrated in the regulation of RNA biosynthesis, cytoplasmic ribosome, and the DNA binding transcription factor activity. In Kyoto Encyclopedia of Genes and Genomes analysis, they are mainly concentrated in TGF-β signal pathway, Notch signaling pathway, cAMP signaling pathway, and Apelin signaling pathway. Gene set enrichment analysis shows that DEGs are mainly enriched in cytoplasmic ribosome, transcriptional regulator activity, DNA binding transcription factor activity, TGF-β signal pathway, and Notch signal pathway. In the enrichment project of Metascape, tyrosine kinase receptor signaling, growth regulation, and enzyme-linked receptor protein signaling pathways can be seen in the gene ontology enrichment project. Four core genes (NOC3L, WDR46, SDAD1, and DDX17) were obtained. Core genes (NOC3L, WDR46, SDAD1, and DDX17) were low expressed in both acquired immunodeficiency and osteoporosis samples. Comparative toxicogenomics database analysis showed that core genes (NOC3L, WDR46, SDAD1, and DDX17) were associated with inflammation necrosis. The expressions of NOC3L and DDX17 are low in acquired immunodeficiency combined with viral myocarditis and osteoporosis.