Lentiviral vectors and protocols for creation of stable hESC lines for fluorescent tracking and drug resistance selection of cardiomyocytes

用于荧光追踪和心肌细胞耐药性选择的稳定 hESC 系的慢病毒载体和方案

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作者:Hiroko Kita-Matsuo, Maria Barcova, Natalie Prigozhina, Nathan Salomonis, Karen Wei, Jeffrey G Jacot, Brandon Nelson, Sean Spiering, René Haverslag, Changsung Kim, Maria Talantova, Ruchi Bajpai, Diego Calzolari, Alexey Terskikh, Andrew D McCulloch, Jeffrey H Price, Bruce R Conklin, H S Vincent Chen, 

Background

Developmental, physiological and tissue engineering studies critical to the development of successful myocardial regeneration therapies require new ways to effectively visualize and isolate large numbers of fluorescently labeled, functional cardiomyocytes. Methodology/principal findings: Here we describe

Significance

The protocols, vectors and gene expression data comprise tools to enhance cardiomyocyte production for large-scale applications.

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