Abstract
During the exploration of microbial natural resources, two strains of Pseudomonas, PS14(T) and PS24(T), were isolated from samples taken from Izu Oshima, a volcanic island located 120 km southwest of central Tokyo. Phylogenetic analysis based on 16S rRNA gene sequences showed that PS14(T) was most similar to Pseudomonas baetica a390(T) (99.6%) and Pseudomonas helmanticensis OHA11(T) (99.5%), and that PS24(T) was most similar to Pseudomonas qingdaonensis JJ3(T) (98.8%) and Pseudomonas lutea OK2(T) (98.7%). The major fatty acids of these two strains were C(16:0) and C(17:0) cyclo, summed feature 3 (C(16:1) ω6c and/or C(16:1) ω7c), and summed feature 8 (C(18:1) ω7c and/or (18:1) ω6c). The phylogenetic analyses, DNA-DNA hybridization results and phenotypic traits indicated that PS14(T) and PS24(T) constitute two novel species, Pseudomonas atagosis sp. nov. (type strain PS14(T) = CECT 9940(T), = LMG 31496(T)) and Pseudomonas akappagea sp. nov. (type strain PS24(T) = CECT 9941(T), = LMG 31497(T)), respectively. The sequence data of the draft genomes of PS14(T) and PS24(T) were deposited in the GenBank database under accession numbers VXCA00000000 and VXCP00000000, respectively, and the sequence data of their 16S rRNA genes were deposited in the GenBank database under accession numbers MN396717 and MN382268, respectively.