Abstract
Despite the intrinsic charge heterogeneity of proteins plays a crucial role in the liquid-liquid phase separation (LLPS) of a broad variety of protein systems, our understanding of the effects of their electrostatic anisotropy is still in its early stages. We approach this issue by means of a coarse-grained model based on a robust mean-field description that extends the DLVO theory to non-uniformly charged particles. We numerically investigate the effect of surface charge patchiness and net particle charge on varying these features independently and with the use of a few parameters only. The effect of charge anisotropy on the LLPS critical point is rationalized via a thermodynamic-independent parameter based on orientationally averaged pair properties, that estimates the particle connectivity and controls the propensity of the liquid phase to condensate. We show that, even though directional attraction alone is able to lower the particle bonding valence-thus shifting the critical point towards lower temperatures and densities-directional repulsion significantly and systematically diminishes the particle functionality, thus further reducing the critical parameters. This electrostatically-driven shift can be understood in terms of the additional morphological constraints introduced by the directional repulsion, that hinder the condensation of dense aggregates.