Partial and complete sequence of small and large subunit ribosomal RNA genes, tRNA-Val gene in some species of family Labridae

隆头鱼科某些物种中小亚基和大亚基核糖体RNA基因以及tRNA-Val基因的部分和完整序列

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Abstract

BACKGROUND: Mitochondrial genomes play a key role in molecular biology research by providing essential information about evolutionary links, population history, and genetic diversity. AIM: The aim of this investigation was to produce a partial sequence of 12S rRNA and 16S rRNA genes, as well as a complete sequence of tRNA-Val gene in some species of family Labridae. METHODS: Five species of labrid fishes (Oxycheilinus digramma, Cheilio inermis, Epibulus insidiator, Coris aygula, and Gomphosus caeruleus) belonging to Family Labridae were collected from the Red Sea, thereafter, taken to a laboratory for morphological identification in accordance with. Using forward and reverse primers, genome DNA was amplified through polymerase chain reaction. RESULTS: The tRNA-Val gene's entire sequence, the 12S rRNA gene's partial sequence, and the 16S rRNA gene's partial sequence were all submitted to GenBank/NCBI with accession numbers (PP962382.1-PP962386.1). The sequences' outcomes showed that the average A + T values were higher than the C + G values. CONCLUSION: The partial sequences of 12S RNA and 16S RNA, and the whole sequence of the tRNA-Val gene, were arranged so that, the 12S RNA and 16S RNA have been distinguished by the tRNA-Val gene.

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