Abstract
A filamentous fungi F. equiseti strain ANP2 with good exopolysaccharide (EPS) yield, apparent viscosity was screened and isolated from Krishna estuarine mangrove sediments. Based on molecular characteristics the selected strain was confirmed as F. equiseti, yielded EPS (4.9 ± 0.25 g/L) and whole cell biomass (3.5 ± 0.2 g/L) in MSM during early stationary phase. The crude EPS was purified by chromatography of Q-Sepharose and Superdex-75, affording Ms and MF-1 fractions were obtained, respectively. Chemical analyses revealed that EPS is primarily composed of neutral sugars and proteins. FTIR-spectroscopy revealed presence of hydroxyl, carbonyl groups and glycosidic bonds which correspond to typical heteropolysaccharide. GC analysis demonstrated that MF-1 mainly consist of mannose (72.6%) and glucose (27.3%). Viscosity studies exhibited typical non-Newtonian pseudoplastic behaviour with high viscosity. The scavenging ability on hydroxyl radicals, indicate that MF-1-EPS has good antioxidant activity. Current study demonstrated that MF-1 differed from previously characterized Fusarium sp., and can serve as potential antioxidant.