Abstract
This study investigated the protective effects of a ceramides derivates from the peach (PF3) on photoaging by UV-irradiated hairless mice. Mice were randomly divided into seven groups: AIN93G without UVB exposure (normal control, NC), AIN93G with UVB exposure (control, C), AIN93G supplemented 100 mg/kg body weight (BW) of L-ascorbic acid with UVB exposure (AA), AIN93G supplemented 100 mg/kg BW of arbutin with UVB exposure (Arbutin), AIN93G supplemented 10 mg/kg BW of PF3 with UVB exposure (10PF3), AIN93G supplemented 20 mg/kg BW of PF3 with UVB exposure (20PF3), and AIN93G supplemented 40 mg/kg BW of PF3 with UVB exposure (40PF3). The study examined the impact of PF3 on skin hydration, wrinkle formation, and melanogenesis using enzyme-linked immunosorbent assay (ELISA), real-time polymerase chain reaction (real-time PCR), and Western blot analysis. The PF3 demonstrated significant protective effects against photoaging by reducing skin wrinkle formation, decreasing epidermal and dermal thickening, and improving skin hydration. It also enhanced the expression of moisture-related factors (hyaluronic acid synthase [HAS], long-chain ceramides [LCBs], dihydroceramide desaturase 1 [DEGS1], and type I collagen [COL1A]) and antioxidant enzyme activities while reducing pro-inflammatory cytokines and oxidative stress markers. The PF3 supplementation positively modulated skin wrinkle formation-related factors, increasing collagen-related gene expression and decreasing matrix metalloproteinases. Additionally, PF3 showed potential in regulating melanogenesis by reducing the nitric oxide and cAMP content, as well as the expression of melanogenesis-related proteins. These comprehensive findings suggest that PF3 supplementation may be an effective strategy for preventing and treating UVB-induced skin photoaging through multiple mechanisms, including improved skin structure, hydration, antioxidant defense, and reduced inflammation and pigmentation.