Abstract
Soil microorganisms play an important role in energy flow and nutrient cycling between soil matrix and plants, but the effect of continuous cultivation on soil bacterial and fungal communities in artificial paddy with lined-film practice remains uncertain. Therefore, we investigated the microbial diversity and community of a soil lined-film with rice grown continuously for a maximum of 27 years. Our results showed that the α-diversity of soil bacterial communities was higher than that of fungi under continuous cultivation. Continuous tillage had a greater effect on the soil bacterial community than on the fungal community, and the fungal community was more stable than the bacterial community. Bacteria were mainly dominated by Actinobacteria, Proteobacteria and Firmicutes, while fungi were dominated by Ascomycota and Basidiomycota. In addition, the fungal Chao 1 index was significantly affected by SOC and TN. Soil pH is an important driver of soil microbial communities under fertilization conditions in sandy artificial paddy field. The results of this study provide empirical support for the sustainable development of lined-film paddy cultivation in sandy farmland ecosystems. They offer a basis for formulating fertilization management strategies that maintain microbial diversity, ensure sustainable land development, and combat desertification.