Abstract
Quantitative gait analysis is essential for understanding motor function and guiding clinical decisions. While marker-based motion capture (MoCap) systems are accurate, they are costly and require specialized facilities. OpenCap, a markerless alternative, offers a more accessible approach; however, its reliability across different walking speeds remains uncertain. This study assessed the agreement between OpenCap and MoCap in measuring spatiotemporal parameters, joint kinematics, and center of mass (CoM) displacement during level walking at three speeds: slow, self-selected, and fast. Fifteen healthy adults performed multiple trials simultaneously, recorded by both systems. Agreement was analyzed using intraclass correlation coefficients (ICC), minimal detectable change (MDC), Bland-Altman analyses, root mean square error (RMSE), Statistical Parametric Mapping (SPM), and repeated-measures ANOVA. Results indicated excellent agreement for spatiotemporal variables (ICC ≥ 0.95) and high consistency for joint waveforms (RMSE < 2°) and CoM displacement (RMSE < 6 mm) across all speeds. However, the joint range of motion (ROM) showed lower reliability, especially at the hip and ankle, at higher speeds. ANOVA revealed no significant System × Speed interactions for most variables, though a significant effect of speed was noted, with OpenCap underestimating walking speed more at fast speeds. Overall, OpenCap is a valuable tool for gait assessment, very accurate for spatiotemporal data and CoM displacement. Still, caution should be taken when interpreting joint kinematics and speed at different walking speeds.