Abstract
Volatile organic compounds (VOCs) are closely associated with the maturity and variety of strawberries. However, the complexity of VOCs hinders their potential application in strawberry classification. This study developed a novel classification workflow using strawberry VOC profiles and machine learning (ML) models for precise fruit classification. A comprehensive VOC dataset was rapidly collected using gas chromatography-ion mobility spectrometry (GC-IMS) from five strawberry varieties at four maturity stages (n = 300) and visualized through principal component analysis (PCA). Five ML models were developed, including partial least squares discriminant analysis (PLS-DA), decision trees, support vector machines (SVM), Xgboost and neural networks (NN). The accuracy of all models ranged from 90.00% to 98.33%, with the NN model demonstrating the best performance. Specifically, it achieved 96.67% accuracy for single-maturity classification, 98.33% for single-variety classification, and 96.67% for dual maturity and variety classification, along with 98.09% precision, 97.92% recall, and 97.91% F1 score. Feature importance analysis indicated that the NN model exhibited the most balanced reliance on various VOCs, contributing to its optimal performance with the broad-spectrum VOC detection method, GC-IMS. Overall, these findings underscore the potential of NN modeling for accurate and efficient fruit classification based on integrated VOC profiles.