Abstract
Arabinoxylan, a key non-starch polysaccharide in wheat bran, significantly influences the quality and health benefits of wheat beer. This study aimed to investigate how wheat bran addition (0-20%) affects water-extracted arabinoxylan (WEAX) content and beer quality in 100% wheat malt beer. The study integrated physicochemical analyses (polysaccharide composition, WEAX molecular weight), process parameters (wort filtration time, foam stability), and sensory evaluation to establish structure-function relationships. Results showed that the WEAX content in beer increased from 1.36 mg/mL in pure malt beer (0% bran) to 2.25 mg/mL with 20% bran addition. Bran addition shortened wort filtration time by 20-45%. The molecular weight of WEAX was mainly 2936-7062 Da, enhancing foam expansion (36.18%) and stability (15.54%) due to elevated polymerization and arabinose-to-xylose (A/X) ratios. WEAX fractions (7062-10,134 Da and 859-2936 Da) correlated positively with beer turbidity and viscosity. Sensory analysis identified 15% bran as optimal for balanced quality. These findings demonstrate that bran addition enhances WEAX content, polymerization, and A/X ratios, improving foam performance, reducing filtration time, and optimizing beer quality without altering arabinogalactan, glucan, or mannose polymer content.