Abstract
In this paper, the mechanism of the Wnt/β-catenin pathway is introduced, and the process and principle of the experiment conducted by Huang et al is explained. We discussed the reliability of the conclusion that Calculus bovis (C. bovis) inhibits M2 tumor-associated macrophage polarization via Wnt/β-catenin pathway modulation to suppress liver cancer. We also offer suggestions for further studies of the use of C. bovis in the treatment of liver cancer.