Abstract
There is increasing interest in foods that support both physical and psychological health. Red and black fruits are notable for their high phenolic content and associated biological activities. However, their natural sugar content may raise concerns regarding glycemic impact. Recent breeding programs have developed new seedless table grape varieties with black skin and red pulp, aiming to enhance phenolic content and reduce glycemic response. This study evaluates these novel grape varieties using in vitro models of intestinal absorption and hepatic insulin resistance. Specifically, we assessed phenolic content, antioxidant capacity, glucose transport across intestinal cells, and the modulation of biomarkers related to insulin resistance. The results showed that the new grape varieties (hybrids) showed total phenolic contents of 52.4-187.3 mg GAE/100 g FW and antioxidant capacities ranging between 195.3 and 762.7 mg Trolox equivalents/100 g FW, both higher than those of commercial table grapes. These new varieties also showed a lower percentage of intestinal glucose transport than commercial grapes and pineapple in caco-2 cells, suggesting an improved regulation of glucose uptake. Theoretical transport values confirmed a reduced glycemic impact for most hybrids, while absorbed fractions of RF03, RF05, and RF06 also restored hepatic glycogen levels under insulin-resistant conditions, indicating enhanced glucose metabolism. Overall, our in vitro findings suggest that these new grape varieties may help modulate postprandial glucose levels, supporting their potential as a healthier fruit option.