Abstract
Acidity in vesicles of macrophages is a general signal that bacteria respond to during infection. Mycobacteria are particularly capable of resisting the acidification in macrophages that engulf the bacteria. In this work, we used label-free quantitative proteomics to study the Mycobacterium smegmatis proteome under acid stress so as to gain an insight into the acidic adaptation in mycobacteria. We quantified 1032 proteins. With a 3-fold change threshold, 20 and 52 proteins were found regulated at false discovery rates of 5% and 14% respectively. We performed a systems analysis based on gene ontology for the global proteome expression profile. We found that the most significant changes induced by the acid stress include a downregulation of transmembrane transporter activity and an upregulation of enzymes involved in fatty acid metabolism. The results suggest that reduced transmembrane transport and increased fatty acid metabolism probably contribute to or associate with acid tolerance in mycobacteria.