Abstract
Rice aroma is influenced by many factors, including selenium (Se) fertilizer. In this study, we investigated the effects of different Se species on the volatile organic compounds (VOCs) in three indica rice varieties over 2022 and 2023 by forliar spray. The VOCs were analyzed using HS-SPME-GC-MS. The results showed that both Se nanoparticles (SeNPs) and sodium selenite (Na(2)SeO(3)) significantly increased the contents of most VOCs in all three varieties, with SeNPs exhibiting a more pronounced effect. PCA and OPLS-DA revealed distinct clustering of the VOCs based on Se treatments and rice varieties. By variable importance in projection (VIP) analysis with FDR correction, Na(2)SeO(3) yielded 7 markers, whereas SeNP treatment identified 18. Every marker detected under Na(2)SeO(3) was fully encompassed within the SeNPs set. Three-factor ANOVA indicated that there are significant interaction effects among Se species, rice variety, and planting year. Additionally, the effect sizes were evaluated in the key VOCs to quantify the effect of Se species, rice variety, and planting year. The findings highlight Se fertilizers to enhance rice aroma and suggest selecting appropriate Se species and rice varieties for aroma improvement.