Abstract
Wood tracheids and fibers exhibit diverse structures and shapes across plant species. The hierarchical structure and composition of cellulose, hemicelluloses, and lignin enables wood to withstand high stress. This structural resilience makes wood a versatile material for applications ranging from construction to advanced composites. However, a detailed understanding of how delignification affects softwood tracheid and hardwood fiber morphology is crucial for predicting material behavior and developing modified wood products. This study investigated the overall structural changes due to delignification, in five wood species, namely, spruce, beech, balsa, Douglas fir, and poplar. It additionally provides detailed morphology of delignified single tracheids and fibers. Scanning electron microscopy was used to compare the morphology between untreated and delignified fibers and tracheids. X-ray tomography enabled us to reconstruct high-resolution 3D models of delignified single tracheids or fibers, providing information on the pit arrangements. Moreover, delignification resulted in facilitated separation of fibers and tracheids and frayed wall appearance. We observed similar tracheid/fiber diameters and wall thicknesses for all five wood species. These findings enhance our understanding of the wood fiber and tracheid structures across species and the effects of delignification. The 3D models provide a valuable resource for (1) understanding interspecies differences of fibers and tracheids, (2) optimizing the use of delignified wood in industrial applications (including bio-based and bio-inspired materials), and (3) physical modeling of wood regarding questions of wood biomechanics and water management.