Abstract
In this study, the traditional fermentation starter (Daqu), a fermentation by-product (Huangshui) and fermentation grains (Zaopei) were combined with pit mud to provide the initial bacterial source, using a single pit mud bacterial inoculation source as the control group. Changes in metabolite accumulation and microbial community were assessed over six rounds of enrichment. Results showed that the addition of exogenous microorganisms (Daqu, Huangshui and Zaopei) better enhanced the quality of the enriched pit mud liquid compared to the use of multiple rounds of enrichment. The quality enhancement of the enriched pit mud liquid could be ranked in descending order as follows: Zaopei > Huangshui > Daqu. The quality of the enriched pit mud liquid was found to be highest in rounds 2-3 of the addition of Zaopei. These results provide theoretical guidance and technical support for the development of pit mud maintenance systems and techniques for the rapid aging of artificial pit mud. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10068-025-01824-z.