Abstract
The phenolic profile of cypress seed extract (CSE) was investigated by means of liquid chromatography with photodiode array and electrospray ionisation mass spectrometric detection (LC/PDA/ESI-MS). The total phenolic (TP) and flavonoid (TF) contents as well as the antioxidant capacity of CSE were determined. The effects of CSE concentration (0.1, 0.3, 0.5, 1, and 2% (w/v)) on the functional properties of hydroxypropylmethylcellulose (HPMC) films were studied. Results showed that CSE presents a good antioxidant capacity due to its high phenolic/flavonoid contents and particularly the presence of bi-flavonoid compounds including cupressuflavone and amentoflavone derivatives. The incorporation of CSE in HPMC films led to a significant decrease in their water vapor permeability (WVP) and enhanced their mechanical strength. The lowest WVP value, the greatest opacity and the highest antioxidant capacity were obtained with the highest CSE concentration. HPMC films with and without CSE were applied on virgin olive oil to study their effect on the oxidative stability of olive oil during accelerated storage by periodically analyzing changes in FTIR spectra and peroxide values. HPMC-2% CSE films were the most effective in lowering light transmission, and consequently decreasing peroxide formation and delaying oxidation of olive oil.