Colorimetric detection of Cu(2+) based on the formation of peptide-copper complexes on silver nanoparticle surfaces

基于肽-铜络合物在银纳米粒子表面形成的比色法检测Cu(2+)

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Abstract

We developed a colorimetric method for the rapid detection of copper ions (Cu(2+)) in aqueous solution. The detection of Cu(2+) is based on coordination reactions of Cu(2+) with casein peptide-functionalized silver nanoparticles (AgNPs), leading to a distinct color change of the solution from yellow to red. The developed method has a good detection limit of about 0.16 µM Cu(2+) using 0.05 mL of AgNPs stock solution and a linearity in the range of 0.08-1.44 µM Cu(2+) with a correlation coefficient of R(2) = 0.973. The developed method is a useful tool for the detection of Cu(2+) ions. Furthermore, it can be used for monitoring Cu(2+) in water at concentrations below the safe limit for drinking water set by the World Health Organization.

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