Structural analysis of conformational changes in the mpox virus A7 protein

• Phospholipid-binding abilities of mpox virus A7 protein and its truncations are investigated. • The structures of the N-terminal truncations of A7 protein (A7N(121) and A7N(137)) are determined. • Conformational changes of the conserved linking helix in A7 are illustrated. • A structural model of the full-length A7 protein is proposed.