Simple and safe method for simultaneous isolation of microbial RNA and DNA from problematic populations

从问题群体中同时分离微生物 RNA 和 DNA 的简单安全方法

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作者:Simon McIlroy, Kate Porter, Robert J Seviour, Daniel Tillett

Abstract

We describe a novel, rapid, and safe method for extracting RNA and DNA from refractory microbes, which avoids the use of phenol or chloroform. It has been used successfully to isolate high-quality nucleic acids from pure cultures and environmental populations known to resist widely used extraction protocols.

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