Abstract
Bashkir Preventing bacterial attachment to surfaces is the most efficient approach to controlling biofilm proliferation. The aim of this study was to compare anti-adhesion potentials of 5 and 50 mmol/L polyelectrolyte multilayers of poly(allylamine hydrochloride)/poly(sodium 4-styrenesulfonate), poly(4-vinyl-N-ethylpyridinium bromide)/ poly(sodium 4-styrenesulfonate), and poly(4-vinyl-N-isobutylpyridinium bromide)/poly(sodium 4-styrenesulfonate) against Escherichia coli. Glass surface was covered with five polyelectrolyte layers and exposed to bacterial suspensions. Poly(4-vinyl-N-ethylpyridinium bromide)/poly(sodium 4-styrenesulfonate) was the most effective against bacterial adhesion, having reduced it by 60 %, followed by poly(4-vinyl-N-isobutylpyridinium bromide)/poly(sodium 4- styrenesulfonate) (47 %), and poly(allylamine hydrochloride)/poly(sodium 4-styrenesulfonate) (38 %). Polyelectrolyte multilayers with quaternary amine groups have a significant anti-adhesion potential and could find their place in coatings for food, pharmaceutical, and medical industry. Preprečevanje adhezije bakterij na površine je najbolj učinkovit način obvladovanja rasti biofilmov. Namen te raziskave je bil analizirati anti-adhezivni potencial 5 in 50 mmol/L polielektrolitskih plasti poli(alilamin hidroklorid)/poli(natrijev 4-stirensulfonat), poli(4-vinil-N-etilpiridin bromid/ poli(natrijev 4-stirensulfonat) in poli(4-vinil-N-izobutilpiridin bromid/ poli(natrijev 4-stirensulfonat) na bakterijo E. coli. Pet zaporednih plasti polielektrolitov je bilo sestavljenih na steklenih površinah in izpostavljenih bakterijski suspenziji. Rezultati kažejo, da 50 mmol/L poli(4-vinil-N-etilpiridin bromid/ poli(natrijev 4-stirensulfonat) najbolj učinkovito prepreči adhezijo bakterij 0,4 log bakt./mm2 (60 %), sledi mu poli(4-vinil-N-izobutilpiridin bromid/ poli(natrijev 4-stirensulfonat) 0,3 log bakt. mm-2 (47 %) in poli(alilamin hidroklorid)/ poli(natrijev 4-stirensulfonat) 0,2 log bakt. mm-2 (38 %). Ta raziskava dokazuje, da polieletrolitske plasti z kvartarne amino skupinami igrajo pomembno vlogo pri preprečevanju adhezije bakterij in zato predstavljajo pomembno uporabo v živilski in farmacevtski industriji ter v medicini.