Abstract
Ligilactobacillus agilis LDTM47 was isolated from gastrointestinal tract of broiler chickens from a farm associated with Chung-Ang University (Anseong, Korea). Ligilactobacillus are Gram-positive lactobacilli associated with the intestinal tracts of vertebrates. Lactic acid bacteria are considered to have a generally rec¬ognized as safe (GRAS) status from the Food and Drug Administration (FDA). The genome of Lig. agilis LDTM47 was 2,144,466 bp long assembled into 1 contig, with 2,131 protein-coding sequences, 90 tRNA genes, 24 rRNA genes, and a guanine + cytosine (GC) content of 41.9%. Strain LDTM47 was selected based on its inhibitory activity against Listeria monocytogenes during isolation. The genome analysis of LDTM47 revealed genes encod¬ing the bacteriocin core peptides and associated export proteins. This study analysis confirmed bacteriocin stability (instability index 1.32) and susceptibility to proteolytic enzymes, indicating non-toxicity. These findings suggest their potential as a safe alternative to antibiotics for controlling pathogens.